Relia Tech/Human Semaphorin 3A/25 µg/100-151
市场价：
¥3400.00
美元价：
2040.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Cat-Nr. | 100-151 |
| Size | 25 µg |
| Price | 170 € |
| Source | CHO cells |
| Label | His-/Fc-tag |
| Formulation | lyophilized |
| Purity Confirmation | > 95% by SDS-PAGE & HPLC analyses |
| Length [aa] | 988 |
| Molecular Weight | >200 kDa (nonreducing conditions) |
| Endotoxin Levels | < 0.1 ng/µg of protein (<1EU/µg) |
| Biological Activity | Determined by its ability to bind recombinant rat Neuropilin-1 Fc Chimera in a functional ELISA assay. |
| Species Reactivity | Human |
| Reconstitution | Centrifuge the vial prior to opening. Reconstitute in 1x PBS to a concentration of 0.1-0.25 mg/ml. NOTE: Allow the reconstituted vial to sit at room temperature for 1 hour before use. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA). |
| Stability and Storage | The lyophilized protein is stable at room temperature for 1 month and at 4°C for 3 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C. |
| Synonyms | SEMA3A, SEMAD |
| Description | Semaphorins are a large group of structurally-related, secreted, GPI-anchored, transmembrane, cell-signaling molecules. There are 8 major classifications of Semaphorins (the first seven ordered by number, 1-7, and the eighth designated V for virus), which are characterized by the existence of a conserved 500 amino acid SEMA domain at the amino terminus. Classes 3, 4, 6, and 7 are found in vertebrates only, whilst class 5 is found in both vertebrates and invertebrates. Each class is then divided into additional subgroups based on shared structural characteristics.Semaphorins primarily function as axon growth cone guidance factors during neuronal development. Semaphorin 3A acts as a chemo-repellent to axons, and an inhibitor of the growth of axons by signaling through receptors, Neuropilin-1 and Plexin-A.The CHO cell-derived Recombinant Human Semaphorin 3A Fc is a glycosylated, disulfide-linked homodimer of 1,976 amino acid residues, which includes the SEMA domain, immunoglobulin c2-like domain, and the C-terminal basic Arg/Lys-rich domain of the mature sequence, as well as an 8-residue N-terminal His-tag and a 230-residue C-terminal Fc region linked by two glycines. Recombinant Human Semaphorin 3A Fc has a calculated molecular weight of 226.2 kDa and therefore runs above the 200kDa marker by SDS-PAGE analysis under nonreducing conditions. When run under reducing conditions, this protein migrates as three distinct bands that, due to glycosylation, run higher than expected at apparent molecular weights of approximately 120-130 kDa, 90-100 kDa, and 35-40 kDa. |
| Protein Sequence | HHHHHHHHGK NNVPRLKLSY KEMLESNNVI TFNGLANSSS YHTFLLDEER SRLYVGAKDH IFSFDLVNIK DFQKIVWPVS YTRRDECKWA GKDILKECAN FIKVLKAYNQ THLYACGTGA FHPICTYIEI GHHPEDNIFK LENSHFENGR GKSPYDPKLL TASLLIDGEL YSGTAADFMG RDFAIFRTLG HHHPIRTEQH DSRWLNDPKF ISAHLISESD NPEDDKVYFF FRENAIDGEH SGKATHARIG QICKNDFGGH RSLVNKWTTF LKARLICSVP GPNGIDTHFD ELQDVFLMNF KDPKNPVVYG VFTTSSNIFK GSAVCMYSMS DVRRVFLGPY AHRDGPNYQW VPYQGRVPYP RPGTCPSKTF GGFDSTKDLP DDVITFARSH PAMYNPVFPM NNRPIVIKTD VNYQFTQIVV DRVDAEDGQY DVMFIGTDVG TVLKVVSIPK ETWYDLEEVL LEEMTVFREP TAISAMELST KQQQLYIGST AGVAQLPLHR CDIYGKACAE CCLARDPYCA WDGSACSRYF PTAKRATRAQ DIRNGDPLTH CSDLHHDNHH GHSPEERIIY GVENSSTFLE CSPKSQRALV YWQFQRRNEE RKEEIRVDDH IIRTDQGLLL RSLQQKDSGN YLCHAVEHGF IQTLLKVTLE VIDTEHLEEL LHKDDDGDGS KTKEMSNSMT PSQKVWYRDF MQLINHPNLN TMDEFCEQVW KRDRKQRRQR PGHTPGNSNK WKHLQENKKG RNRRTHEFER APRSVGGPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK |
| Uniprot ID | Q14563 |
| Protein RefSeq | NP_006071.1 |
| mRNA RefSeq | NM_006080.2 |
品牌介绍
Receptor Ligand Technologies GmbH 公司（RELIA Tech ）位于德国不伦瑞克，是一家后基因组生物技术公司，该公司运用独特的技术专注于受体和配体的发现与研究，从而使商业化，产品广泛使用于科学研究，实验诊断和临床应用。瑞莱技术的灵活性和竞争力必将使其在这个快速整合的功能基因组学和蛋白组学的新时代发展壮大.RELIA公司提供的细胞因子，生长因子，重组蛋白产品一致内毒素检测项目，且内毒素水平非常低。 受体配体技术有限公司（RELIATech）是一家后基因组生物技术公司，致力于在配体与受体相互作用领域的新技术和新产品的发现和商业化，用于研究，诊断和临床领域。该公司位于德国不伦瑞克，由以下公司成立于2000年10月： 阿夫纳·亚永教授以色列魏兹曼科学研究所（WIS） 赫伯特·韦奇博士德国生物技术德国研究中心（GBF） Bernhard Barleon博士德国肿瘤生物学诊所（KTB） 创始人是从事细胞生长因子相互作用，酪氨酸激酶及其信号传导途径（涉及组织重塑，体内平衡和疾病）领域的细胞和分子生物学家，工作时间超过10年。特别是Herbert A. Weich博士和Bernhard Barleon博士从事血管生成，肿瘤血管生成和实体瘤进展领域的基础研究。 诸如“生长因子和细胞因子”之类的信号蛋白，连同其跨膜/“可溶性受体”和相关的激酶，在生理和病理生理条件下都作为关键的调节分子参与了所有高级生物的发育和功能。这些多肽调节细胞分化，组织重塑和体内平衡，并在各种疾病状态下被失调。由人类基因组计划编码新信号蛋白的新生长调节基因的发现，以及对它们功能的阐明，无疑将是未来十年的主要挑战之一。 RELIATech的使命是开发和商品化在“受体和配体相互作用”领域中有用的新产品，用于基础研究，药物开发和临床社区。RELIATech的灵活性和能力必将使其在功能基因组学和蛋白质组学的新时代迅速整合和扩展。
联络我们
